Antagonists of DNA gyrase inhibit repair and recombination of UV-irradiated phage lambda.
نویسندگان
چکیده
منابع مشابه
DNA structures generated during recombination initiated by mismatch repair of UV-irradiated nonreplicating phage DNA in Escherichia coli: requirements for helicase, exonucleases, and RecF and RecBCD functions.
During infection of homoimmune Escherichia coli lysogens ("repressed infections"), undamaged nonreplicating lambda phage DNA circles undergo very little recombination. Prior UV irradiation of phages dramatically elevates recombinant frequencies, even in bacteria deficient in UvrABC-mediated excision repair. We previously reported that 80-90% of this UvrABC-independent recombination required Mut...
متن کاملDirectionality and nonreciprocality of Chi-stimulated recombination in phage lambda.
Chi's are genetic elements that stimulate generalized recombination in their locale in phage lambda. All Chi's wherever located on lambda's chromosome, act asymmetrically in crosses blocked in DNA replication: (1) They stimulate exchange primarily to their left on the conventional lambda map, and (2) the stimulated exchange is frequently nonreciprocal, the recombinant carrying the Chi element b...
متن کاملU.V. induced enhancement of recombination among lambda bacteriophages: relation with replication of irradiated DNA.
Experimental results are reported showing the dependence of the U.V. induced enhancement of recombinants on the presence of the functional O gene product. This fact is tentatively interpreted as a replication dependence of the U.V. induced recombination.
متن کاملThe mechanism of phage lambda site-specific recombination: site-specific breakage of DNA by Int topoisomerase.
We demonstrate that the topoisomerase activity of bacteriophage lambda Int protein introduces single-strand breaks into duplex DNA at specific sites. Strand breakage is accompanied by the covalent linkage of Int to DNA. The linkage connects a residue in Int to the 3' phosphate of DNA at the site of breakage; the other breakage product has a 5' OH terminus. Int is the first procaryotic topoisome...
متن کاملNovobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.
Novobiocin and coumermycin are known to inhibit the replication of DNA iing of DNA catalyzed by E. coli DNA gyrase, a recently discovered enzyme that introduces negative superhelical turns into covalently circular DNA. The activity of DNA gyrase purified from a coumermycin-resistant mutant strain is resistant to both drugs. The inhibition by novobiocin of colicin E1 plasmid DNA replication in a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1978
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.75.9.4125